Department of Microbiology
Special Clinical / Diagnostic services
Infectious diseases continue to be the most common ailment for which an individual visits a hospital. The department of Microbiology identifies the microbial organism responsible for the ailment and also puts up antibiotic susceptibility test to facilitate rational choice of antibiotic by the physicians. Infectious diseases may be trivial such as common cold for which a laboratory diagnosis is not sought, or serious, for which a laboratory diagnosis is pertinent. Some of the major infectious diseases for which a laboratory diagnosis is sought are Tuberculosis, Cholera, Typhoid, AIDS, Viral Hepatitis, Dengue, Urinary Tract Infections, Endocarditis, Rheumatic fever, scrub typhus, etc to name a few. For these infectious, the best diagnostic method is available in our laboratory, namely – culture / serology/ molecular methods.
During the course of MBBS a student of Microbiology, learns the basics of every infectious disease as to its pathogenesis, diagnosis, management and specific prevention with vaccinations.
Apart from routine culture and sensitivity of bacteria and fungi and serological tests for diagnosis of infectious diseases, the laboratory has the following facilities also
Facilities
Diagnostic Laboratory:
Hospital Central laboratory is situated in the ground floor of the Hospital. The investigations carried out in the department of Microbiology are as follows:
Clinical Bacteriology:
The following specimens are received from outpatients and inpatients of the hospital for culture. Blood culture:
- Preparation of Cultural media for culture of the Clinical samples, UG & PG Academic Work.
- Culture for different specimens.
- Respiratory – Throat Swab
- Exudates & Body Fluids
- Urine – Semiquantitative method is done;
- Stool
- Organisms are identified by conventional biochemical and other tests, and antibiotic sensitivity testing is done by disc diffusion method.
- Anaerobic cultures
- Blood Cultures
Serological tests:
- Widal – For enteric fever. Take agglutination test. Commercial kits are used.
- RPR, VDRL – Syphilis;
- ASO Titre
- CRP detection
- RF detection are carried out by Latex agglutination test
- ELISA is carried out for
- Dengue – Rapid ELISA
- Chicken Guinea
- Malaria
- Rapid Tests
- Rapid Tests
- HIV
- HCV
- HbsAg
Mycology:
- KOH mount for fungal elements by conventional methods.
- India ink preparation for cryptococcus.
- Fungal Culture for Conventional Versatresk.
Mycobacteriology:
- Direct smear for Acid fast bacilli.
- L.J Medium for Conventional culture – Under trial.
Parasitology:
- Stool examination for ova or cyst is done by normal Saline and Lugol’s iodine preparation. Modified acid fast staining – for cryptosporidium.
Theatre surveillance is done for all the operation theatres once in a month:
- Settle plate method
- Culture of Swabs – Anaerobic
Water Bacteriology:
- Water samples from different sites in the Hospital and Hospital campus are periodically checked for faecal pollution.
Special Microscopy:
- Dark ground microscopy is done for visualizing Leptospites in the clinical specimens and report is give in correlation with serology.
Fluorescent Microscopy:
- The staining procedures are being standardized.
Virology and PCR sections:
- VRDL – RTPCR for Covid-19
- Viral load – HIV
List of Equipments
- Monocular Microscope (MVTEX, QUASMO) – 141
- Binocular Microscope (OLYMPUS, LABOMED, MAGNUS) – 8
- Fluorescent Microscope – 1
- Incubator (KEMI, BIOTECHNICS) – 3
- Hot Air Oven – 3
- Water Bath – 1
- Hot Plate – 1
- Centrifuge (Remi, EPPENDORF, THERMOFISHER) – 7
- BioSafety Cabinet – 5
- Laminar Air Flow Cabinet – 4
- PCR – Quant Studio – 4
- PCR – Bio-Rad – 3
- Genfast Automated DNA/RNA Extraction System – 3
- Automated NA Extraction System (Hi media) – 1
- Sample Preparation device (TruePrepMag) – 1
- Micro PCR Analyzer (TRULABUNO) – 1
- Refrigerator – 1
- Deep freezer (Remi) – 2
- Ultra low Freezer – 3
- Plate Centrifuge – 1
- Spin Coloumn Centrifuge – 2
- Mastercycler – 1
- Bio- Print Fixed Container (Vilber) – 1
- Thermo Shaker Incubator – 1
- ELISA Plate Reader – 1
- ELISA Plate Washer – 1
- CO2 Incubator (EPPENDORF) – 1
- BOD Incubator – 1
- Autoclave – 1
- Sterilizer – 1
- Distilled Water Purification System (Pall) – 1
- Water distillation Unit – 2
- Vortex Mixer – 1
- Pipette – 24
- Pipette Stand – 2
| Sl. No. | Name of the Faculty | Designation | Qualification | APMC Registration Number |
| 1 | N Padma Priya | Professor | M.D. | 38892 |
| 2 | Angothu Ankamma | Associate Professor | M.D. | 40377 |
| 3 | Sulakshana Sony Cheemala | Assistant Professor | M.D. | 71905 |
| 4 | Vuppalapati Vijaya Swetha | Assistant Professor | M.D. | APMC/64290 |
| 5 | Yeedulamudi Rajani | Assistant Professor | M.D. | 18000 |
| 6 | Ganesh Yadlapalli | Tutor | M.Sc. | NA |
ONGOING RESEARCH PROJECTS
S.NO | TITLE OF ARTICLE | NAME OF AUTHORS |
1 | Correlation of Dengue and Chikungunya infections in a tertiary care hospital | Dr Y Rajani (Assistant Professor) |
2 | Seroprevalence of Leptospirosis in tertiary care hospital in A.P. India | Dr Ch. Sulakshana Sony (Assistant Professor) |
3 | Seroprevalence of Scrub typhus in tertiary care hospital in A.P. India | Dr V V Swetha (Assistant Professor) |
4 | Isolation, Identification, Speciation and Antifungal susceptibility of Candida from various clinical samples in tertiary care hospital | Dr P Shafiya( 3rd Year Post Graduate) Dr N Padma Priya(Professor & HOD) Dr Y Rajani(Assistant Professor) |
5 | Comparison of efficacy of rapid test versus ELISA method for detection of Ns1, IgM, and IgG antibodies in dengue viral infection in a tertiary care hospital | Dr I Divya Sri( 3rd Year Post Graduate) Dr N Padma Priya(Professor & HOD) Dr Y Rajani(Assistant Professor) |
6 | Aerobic bacteriological profile of chronic Suppurative Otitis Media and its antibacterial susceptibility testing in a tertiary care hospital | Dr Preetha S R( 3rd Year Post Graduate) Dr N Padma Priya(Professor & HOD) Dr Y Rajani(Assistant Professor) |
7 | Phenotypic detection of extended spectrum Beta – lactamase, AmpC, Metallobetalactamase in all isolated Gram negative bacteria from various clinical samples in a tertiary care hospital | Dr.Priyam Krishnatreya (2nd Year Post Graduate) Dr N Padma Priya(Professor & HOD) Dr. Angotu Ankamma(Associate Professor) |
8 | Aerobic bacterial and fungal etiological study of secondary infections in patients with Hansen’s diseases in a tertiary care hospital | Dr.Swathy S.R(2nd Year Post Graduate) Dr N Padma Priya(Professor & HOD) Dr. Angotu Ankamma(Associate Professor) |
9 | Bacteriological profile of Uropathogens and their antimicrobial susceptibility testing among pregnant women in a tertiary care hospital | Dr J Narmada(2nd Year Post Graduate) Dr N Padma Priya(Professor & HOD) Dr. Angotu Ankamma(Associate Professor) |
10 | A study of bacteriological profile and antibiogram pattern of wound infection in a tertiary care hospital | Dr. S. Zamsheer Syed (2nd Year Post Graduate) Dr N Padma Priya(Professor & HOD) Dr. Angotu Ankamma(Associate Professor) |
11 | Study of resistance pattern of Clindamycin in MRSA and its relation to MIC amongst clinical samples isolated in a tertiary care hospital | Dr.Priyam Krishnatreya (2nd Year Post Graduate) Dr N Padma Priya(Professor & HOD) |
12 | A study on the aerobic microbiological profile of an abnormal vaginal discharge among the women of reproductive age group and their anti-microbial susceptibility patterns in a tertiary care hospital | Dr. J Narmada(2nd Year Post Graduate)
Dr V V Swetha(Assistant Professor) |
13 | Study of isolation of Pseudomonas aeruginosa and its antibiotic susceptibility pattern from various samples in a tertiary care hospital | Dr. S. Zamsheer Syed (2nd Year Post Graduate) Dr Y Rajani(Assistant Professor) |
14 | A study of prevalence of dermatophytes and their isolation and identification among the patients attending a tertiary care hospital | Dr. V. Avinash Kumar (1st Year Post Graduate) Dr. N Padma Priya(Professor & HOD) Dr. Angotu Ankamma(Associate Professor) |
15 | A study of aerobic bacteriological profile in diabetic foot and its antibiogram among patients attending a tertiary care hospital | Dr. Shehanaz N(1st Year Post Graduate) Dr N Padma Priya(Professor & HOD) Dr. Angotu Ankamma(Associate Professor) |
16 | A study of co-infection of Hep-B & Hep – C among HIV patents attending at tertiary care Hospital | Dr. V. Avinash Kumar, (1st Year Post Graduate) Dr. V V Swetha, (Assistant Professor) Dr. N Padma Priya(Professor & HOD) |
17 | Comparison of UTI in catheterized and non- catheterized patients attending a tertiary care hospital | Dr. Shehanaz N, (1st Year Post Graduate) Dr Ch. Sulakshana Sony (Assistant Professor) Dr. N Padma Priya(Professor & HOD) |
COMPLETED RESEARCH PROJECTS
S.NO | TITLE OF ARTICLE | NAME OF AUTHORS |
1 | A study on prevalence of superficial mycoses and its anti-fungal susceptibility pattern in patients in a tertiary care hospital | Dr Sravanthi(Postgraduate) Dr. B Venkata Rao (Professor & HOD) |
2 | Aerobic microbiological profile of neonatal septicemia in NICU | Dr M Sumani(Post Graduate) Dr. N Padma Priya (Professor & HOD) |
3 | Study of Toxoplasma gondii seropositivity in HIV positive patients with special reference to CD4 count in tertiary care hospital | Dr T Mounica(Post Graduate) Dr. N Padma Priya (Professor & HOD) |
4 | Study of aerobic bacteriological profile of chronic pyogenic osteomyelitis and antibiotic susceptibility in tertiary care hospital | Dr Jagan V(Post Graduate) Dr. N Padma Priya (Professor & HOD) |
5 | Significance of CBNAAT method for diagnosis of Mycobacterium Tuberculosis in tertiary care hospital | Dr. D V Vinay Kumar (Post Graduate) Dr. N Padma Priya (Professor & HOD) |
6 | Pseudomonas aeruginosa-A notorious pathogen for critically ill patients on mechanical ventilator | Dr Jagan V(Post Graduate) Dr. V V Swetha (Assistant Professor) |
7 | Prevalence of COVID 19 infection in vaccinated and infected individual-Knowledge based questionnaire survey | Dr M Sumani(Post Graduate) Dr. P. Queeni Leena, (Assistant Professor) |
8 | Antibiotic susceptibility pattern of bacteria isolated from surgical site infection in a tertiary care center | Dr T Mounica(Post Graduate) Dr. Ch Sireesha (Assistant Professor) |
9 | Hand hygiene compliance of health care providers in a tertiary care hospital | Dr. D V Vinay Kumar (Post Graduate) Dr. D Lavanya, (Assistant Professor) |
10 | Study on prevalence of TB and detection of Rifampicin resistance by CBNAAT and LPA in presumptive pulmonary TB and presumptive pulmonary DRTB patients attending at tertiary care hospital | Dr.I. Divya Sri (3rd Year Post Graduate) Dr. P. Queeni Leena (Assistant Professor) Dr.N Padma Priya (Professor & HOD) |
11 | Isolation and identification of ESBL’s producing Gram –negative bacilli from various samples in a tertiary care hospital | Dr. Preetha S R (3rd Year Post Graduate) Dr. V V Swetha, (Assistant Professor) Dr.N Padma Priya (Professor & HOD) |
12 | A questionnaire based study of microbiology sample collection, transportation, and storage and its knowledge, attitude and skills, among interns at GGH, Ongole. | Dr P Shafiya, (3rd Year Post Graduate) Dr. N Padma Priya (Professor & HOD) |
13 | Bacteriologicalprofile of asymptomatic urinary tract infection with antibiogram from medical ward in a tertiary care hospital. | Dr I Divya sri, (3rd Year Post Graduate) Dr. P. Queeni Leena, (Assistant Professor) Dr.N Padma Priya (Professor & HOD) |
14 | Prevalence of HBV and HCV among blood donors attending blood bank in a tertiary care Centre | Dr. Preetha S R (3rd Year Post Graduate) Dr. V V Swetha, (Assistant Professor) Dr.N Padma Priya (Professor & HOD) |
15 | Awareness and attitude regarding Biomedical waste disposal among post graduates, house surgeons and nursing staff of tertiary care hospital in Ongole | Dr.J Narmada, (2nd Year Post Graduate) Dr. P. Queeni Leena, (Assistant Professor) Dr.N Padma Priya (Professor & HOD) |
16 | Study of prevalence of MRSA from anterior nares of medical students in tertiary care hospital | Dr. Zamsheer Syed, (2nd Year Post Graduate) Dr. V V Swetha, (Assistant Professor) Dr.N Padma Priya (Professor & HOD) |
17 | Study of aerobic bacterial profile and its antibiogram amongst sputum samples of LRTI patients attending a tertiary care hospital | Dr.Priyam Krishnatreya (2nd Year Post Graduate) Dr.N Padma Priya (Professor & HOD) |
18 | Study of bacterial profile and their antibiogram isolated from wound infection at a tertiary care hospital, Ongole | Dr Swathy S R, (2nd Year Post Graduate) Dr. D.Lavnaya, (Assistant Professor) Dr. N Padma Priya (Professor & HOD) |
LIST OF THE WRITE-UP'S
TOPIC | BATCH | NAME OF THE AUTHOR |
Travel medicine and infectious diseases | 2nd year | Dr.Priyam Krishnatreya |
Diagnostic challenges due to emerging antifungal resistance | 2nd year | Dr. Narmada |
Emerging resistance of Anti parasitic agents | 2nd year | Dr. Zamsheer Syed |
Role of Diagnostic Stewardship in combating AMR | 1st year | Dr. Avinash |
Role of multiplex PCR in Syndromic diagnostic testing | 1st year | Dr. Shehanaz |
PUBLISHED ARTICLES
S.NO | TITLE OF ARTICLE | NAME OF AUTHORS | NAME OF JOURNAL |
1 | Genome Analysis for Sequence Variants in Sars-Cov -2 in Symptomatic Individuals at Tertiary Care Hospital | Dr. Mary Sumani Dr. Lavanya. D Dr. Padma Priya. N Dr. QueeniLeena. P, Dr. Vijaya Swetha.V Volume 09, Issue 07, 2022 | European Journal of Molecular & Clinical Medicine |
2 |
Retrospective Study of Efficacy of TLE &TLD Regimens in Correlation with CD4 Count and Viral Load in newly Diagnosed HIV Infected Patients | Dr. Jagan V Dr.V. Vijaya Swetha Dr.N. Padma Priya Volume 09, Issue 07, 2022 | European Journal of Molecular & Clinical Medicine |
3 |
Comparative Analysis of Ns1 Antigen and IGM Antibody by ELISA of Clinically Suspected Dengue Fever Cases in a Tertiary Care Centre | Dr.T. Mounica Dr.D. Lavanya Dr.N. Padma Priya Volume 09, Issue 07, 2022 | European Journal of Molecular & Clinical Medicine |
4 |
Incidence of Tuberculosis in HIV Patients and Its Co-Relation to CD4 Count” – A Retrospective Study at Art Center in Tertiary Care hospital | Dr.D.V. Vinay Kumar Dr.P. Queeni Leena Dr.N.Padama Priya Volume 09, Issue 07, 2022 | European Journal of Molecular & Clinical Medicine |
5 | Post covid-19 fungal infections assessment among patients Attending a tertiary care hospital | Dr.P.Queeni Leena Dr.B.Venkata Rao Volume – 11 | Issue – 01 | January – 2022 | PRINT ISSN No. 2277 – 8179 | DOI : 10.36106/ijsr | International journal of scientific research |
6 | Prevalence of vancomycin resistant enterococcus in various clinical specimens in tertiary care hospital. | Dr. P Shafiya, Dr P. Queeni Leena, Dr. Y Rajani, Dr V V Swetha, Dr. N Padma Priya Volume 13, issued 05, may2024 | International journal of science and research (IJSR) |
7 | Detection of Rifampicin resistance in pulmonary tuberculosis by molecular methods in a tertiary care hospital | Dr. I Divya sree, Dr. N Padma Priya, Dr Sireesha Chava, Dr Sulakshana Sony Cheemala Volume 13, issued 05, may2024 | International journal of science and research (IJSR) |
8 | Isolation, identification, prevalence of ESBL’s producing Gram negative Bacilli from urine and exudate samples in a tertiary care hospital – An observational study | Dr. Preetha S R, Dr Y.Rajani, Dr. D lavanya, Dr Sireesha Chava, Dr N Padma Priya Volume 13, issued 05, may2024 | International journal of science and research (IJSR) |