Department of Microbiology

Special Clinical / Diagnostic services

Infectious diseases continue to be the most common ailment for which an individual visits a hospital. The department of Microbiology identifies the microbial organism responsible for the ailment and also puts up antibiotic susceptibility test to facilitate rational choice of antibiotic by the physicians. Infectious diseases may be trivial such as common cold for which a laboratory diagnosis is not sought, or serious, for which a laboratory diagnosis is pertinent. Some of the major infectious diseases for which a laboratory diagnosis is sought are Tuberculosis, Cholera, Typhoid, AIDS, Viral Hepatitis, Dengue, Urinary Tract Infections, Endocarditis, Rheumatic fever, scrub typhus, etc to name a few. For these infectious, the best diagnostic method is available in our laboratory, namely – culture / serology/ molecular methods.

During the course of MBBS a student of Microbiology, learns the basics of every infectious disease as to its pathogenesis, diagnosis, management and specific prevention with vaccinations.

Apart from routine culture and sensitivity of bacteria and fungi and serological tests for diagnosis of infectious diseases, the laboratory has the following facilities also

Facilities

Diagnostic Laboratory:

Hospital Central laboratory is situated in the ground floor of the Hospital. The investigations carried out in the department of Microbiology are as follows:

• Clinical Bacteriology:

The following specimens are received from outpatients and inpatients of the hospital for culture. Blood culture:

• • Preparation of Cultural media for culture of the Clinical samples, UG & PG Academic Work.
  • Culture for different specimens.
  • Respiratory – Throat Swab
  • Exudates & Body Fluids
  • Urine – Semiquantitative method is done;
  • Stool
  • Organisms are identified by conventional biochemical and other tests, and antibiotic sensitivity testing is done by disc diffusion method.
  • Anaerobic cultures
  • Blood Cultures

• Serological tests:

  • • Widal – For enteric fever. Take agglutination test. Commercial kits are used.
    • RPR, VDRL – Syphilis;
    • ASO Titre
    • CRP detection
    • RF detection are carried out by Latex agglutination test
    • ELISA is carried out for
      • Dengue – Rapid ELISA
      • Chicken Guinea
      • Malaria
      • Rapid Tests
    • Rapid Tests
      • HIV
      • HCV
      • HbsAg
  •  

• Mycology:

  • KOH mount for fungal elements by conventional methods.
  • India ink preparation for cryptococcus.
  • Fungal Culture for Conventional Versatresk.

• Mycobacteriology:

  • Direct smear for Acid fast bacilli.
  • L.J Medium for Conventional culture – Under trial.

• Parasitology:

  • Stool examination for ova or cyst is done by normal Saline and Lugol’s iodine preparation. Modified acid fast staining – for cryptosporidium.

• Theatre surveillance is done for all the operation theatres once in a month:

  • Settle plate method
  • Culture of Swabs – Anaerobic

• Water Bacteriology:

  • Water samples from different sites in the Hospital and Hospital campus are periodically checked for faecal pollution.

• Special Microscopy:

  • Dark ground microscopy is done for visualizing Leptospites in the clinical specimens and report is give in correlation with serology.

• Fluorescent Microscopy:

  • The staining procedures are being standardized.

• Virology and PCR sections:

  • VRDL – RTPCR for Covid-19
  • Viral load – HIV

List of Equipments

• Monocular Microscope (MVTEX, QUASMO) – 141
• Binocular Microscope (OLYMPUS, LABOMED, MAGNUS) – 8
• Fluorescent Microscope – 1
• Incubator (KEMI, BIOTECHNICS) – 3
• Hot Air Oven – 3
• Water Bath – 1
• Hot Plate – 1
• Centrifuge (Remi, EPPENDORF, THERMOFISHER) – 7
• BioSafety Cabinet – 5
• Laminar Air Flow Cabinet – 4
• PCR – Quant Studio – 4
• PCR – Bio-Rad – 3
• Genfast Automated DNA/RNA Extraction System – 3
• Automated NA Extraction System (Hi media) – 1
• Sample Preparation device (TruePrepMag) – 1
• Micro PCR Analyzer (TRULABUNO) – 1
• Refrigerator – 1
• Deep freezer (Remi) – 2
• Ultra low Freezer – 3
• Plate Centrifuge – 1
• Spin Coloumn Centrifuge – 2
• Mastercycler – 1
• Bio- Print Fixed Container (Vilber) – 1
• Thermo Shaker Incubator – 1
• ELISA Plate Reader – 1
• ELISA Plate Washer – 1
• CO2 Incubator (EPPENDORF) – 1
• BOD Incubator – 1
• Autoclave – 1
• Sterilizer – 1
• Distilled Water Purification System (Pall) – 1
• Water distillation Unit – 2
• Vortex Mixer – 1
• Pipette – 24
• Pipette Stand – 2

Department Of Microbiology
S. No.	Name	Designation
1	Dr. N. Padmapriya	Associate Professor & I/C HOD
2	Dr. D. Lavanya	Assistant Professor
3	Dr. P. Queeni Leena	Assistant Professor
4	Dr. Ch. Sireesha	Assistant Professor
5	Dr. V. Vijaya Swetha	Assistant Professor
6	Dr. Y. Ganesh	Tutor